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Influence of body size on fecundity and sperm management in the parasitoid wasp Anisopteromalus calandrae 下载免费PDF全文
A large body size is considered to be advantageous to the reproductive success of females as a result of several factors, such as the allocation of more resources to reproduction and the efficient management of sperm transferred by males. In the present study, the effects of female body size, female mating status and additional food availability on fecundity and the offspring sex ratio are investigated in the parasitoid wasp Anisopteromalus calandrae Howard (Hymenoptera: Pteromalidae). Because of haplodiploid sex determination, females must fertilize eggs to produce female offspring but not to produce male offspring. As predicted, female fecundity and the number of female offspring are positively correlated with body size. However, although the volume of the spermatheca increases with female body size, the amount of sperm stored in the spermatheca is relatively constant, irrespective of body size. Consequently, larger females produce a greater proportion of male offspring, especially at the end of the oviposition sequence, suggesting that larger females that possess more resources for reproduction and produce a larger number of offspring are more likely to suffer sperm depletion. The results of the present study also show that mated females have an increased fecundity compared with virgin females, although the opportunity to feed on honey along with host feeding has no impact upon fecundity or the sex ratio. 相似文献
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Taro Kato Nobutaka Someya Tsukasa Ikeda 《Bioscience, biotechnology, and biochemistry》2013,77(4):673-680
Pseudomonas sp. StFLB209 was isolated from potato leaf as an N-acylhomoserine lactone (AHL)-producing bacterium and showed a close phylogenetic relationship with P. cichorii, a known plant pathogen. Although there are no reports of potato disease caused by pseudomonads in Japan, StFLB209 was pathogenic to potato leaf. In this study, we reveal the complete genome sequence of StFLB209, and show that the strain possesses a ppuI-rsaL-ppuR quorum-sensing system, the sequence of which shares a high similarity with that of Pseudomonas putida. Disruption of ppuI results in a loss of AHL production as well as remarkable reduction in motility. StFLB209 possesses strong pectate lyase activity and causes maceration on potato tuber and leaf, which was slightly reduced in the ppuI mutant. These results suggest that the quorum-sensing system is well conserved between StFLB209 and P. putida and that the system is essential for motility, full pectate lyase activity, and virulence in StFLB209. 相似文献
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Summary Aspects of the reproductive biology of the giant isopod, Bathynomus giganteus (Edwards) resemble those of other isopods. In females, the gonopores are located on the sternal midline of the eighth thoracic somite and the eggs are brooded in a marsupium. The reproductive tract of the males also resembles those of other isopods. The paired vasa deferentia open into two penes located on the sternal midline of the eighth thoracic somite. The vasa deferentia are formed of columnar epithelial cells with basal nuclei. The lumen is filled with seminal products consisting of aggregrates of spermatozoa surrounded by extracellular tubules. The sperm head consists of an acrosome and subacrosomal rod from which a pendant nucleus extends. The tails are composed of an amorphous core consisting of a dark band, two medium bands, two light bands followed by a dark band again. The tails are attached to the heads by a knob which is an extension of the core 相似文献
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《European journal of cell biology》2022,101(3):151241
The viscoelastic parameters of the cell can report on the cell state, cellular processes and diseases. Cell mechanics strongly rely on the properties of the cytoskeleton, an important system of subcellular filaments, especially on the high-level structures that actin forms together with actin-binding proteins (ABPs). In normal cells, components of the cytoskeleton are highly integrated, and their functions are well orchestrated. In contrast, impaired expression and functioning of ABPs lead to the increasing ability of cancer cells to resist chemotherapy and metastasize. ABP-mediated changes in the cytoskeleton architecture can lead to changes in the mechanical properties of the actin network, both locally and at the level of the whole cell. Until now, in cancer-related studies, mechanical data have been used less frequently, compared to biochemical tests or cell migration assays. Here, we will review current methods for analyzing the mechanical properties of cells and provide the available data on the contribution of ABPs in determining cell mechanical properties important for the investigation of cellular functions, particularly in cancers. 相似文献
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This study examined whether the soya phyto-oestrogen genistein would have an effect on spermatozoa quality and in vitro fertilization capacity in mature channel catfish Ictalurus punctatus or walleye Sander vitreus . For both species, motility time and motility rank were significantly different among treatment groups and control, with higher genistein concentrations producing significantly lower motility time and motility rank ( P ≤ 0·01). Walleye and channel catfish ATP content was significantly lower compared to control treatments at several incubation concentrations and was significantly related to fertilization rate. Fertilization rate was significantly dependant on genistein incubation concentrations ( P ≤ 0·01). Additionally, logistic regression showed a significant negative relation between genistein concentration and fertilization in channel catfish ( P ≤ 0·01). These results establish that genistein could play a role in reproductive performance within aquaculture species. In addition, these findings warrant further examination of the impact of phyto-oestrogens in broodstock feeds. 相似文献
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Pseudomonas aeruginosa is capable of moving by swimming, swarming, and twitching motilities. In this study, we investigated the effects of fatty acids on Pseudomonas aeruginosa PAO1 motilities. A branched-chain fatty acid (BCFA)--12-methyltetradecanoic acid (anteiso-C15:0)--has slightly repressed flagella-driven swimming motility and completely inhibited a more complex type of surface motility, i.e. swarming, at a concentration of 10 microg mL(-1). In contrast, anteiso-C15:0 exhibited no effect on pili-mediated twitching motility. Other BCFAs and unsaturated fatty acids tested in this study showed similar inhibitory effects on swarming motility, although the level of inhibition differed between these fatty acids. These fatty acids caused no significant growth inhibition in liquid cultures. Straight-chain saturated fatty acids such as palmitic acid were less effective in swarming inhibition. The wetness of the PAO1 colony was significantly reduced by the addition of anteiso-C15:0; however, the production of rhamnolipids as a surface-active agent was not affected by the fatty acid. In addition to motility repression, anteiso-C15:0 caused 31% repression of biofilm formation by PAO1, suggesting that BCFA could affect the multiple cellular activities of Pseudomonas aeruginosa. 相似文献
20.
《Cell cycle (Georgetown, Tex.)》2013,12(22):3834-3840
MET, a receptor protein tyrosine kinase activated by hepatocyte growth factor (HGF), is a crucial determinant of metastatic progression. Recently, we have identified p53 as an important regulator of MET-dependent cell motility and invasion. This regulation occurs via feedforward loop suppressing MET expression by miR-34-dependent and -independent mechanisms. Here, by using Dicer conditional knockout, we provide further evidence for microRNA-independent MET regulation by p53. Furthermore, we show that while MET levels increase immediately after p53 inactivation, mutant cells do not contain active phosphorylated MET and remain non-invasive for a long latency period at contrary to cell culture observations. Evaluation of mouse models of ovarian and prostate carcinogenesis indicates that formation of desmoplastic stroma, associated production of HGF by stromal cells and coinciding MET phosphorylation precede cancer invasion. Thus, initiation mutation of p53 is sufficient for preprogramming motile and invasive properties of epithelial cells, but the stromal reaction may represent a critical step for their manifestation during cancer progression. 相似文献